What is the name of doctor, professor B. Romeis?

In one paper I found a mention of professor B. Romeis, who wrote a book on "Mikroskopische Technik" (1936). I need to mention him in my paper, but I cannot find his full name anywhere. Even in...

13 January 2021 7,402 3 View

After extracting RNA with AllPrep RNA/DNA/miRNA Universal kit, can DNase digestion in solution with heat inactivation damage miRNA/lower yield?

16 September 2020 7,096 3 View

How to improve Desmond MD/metadynamics calculations performance?

Which program parameters is useful to improve calculations performance (ns/day) of MD simulations without simulation quality loss?

28 June 2020 4,840 2 View

Does it make sense to model mass spectrometric fragmentation by DFT?

Hello. I have met a significant number of publications on the study of the laws of mass fragmentation using DFT. Researchers are building huge schemes where they show in which direction the decay...

05 February 2020 6,252 3 View

How to solve problem with momentum overflow in desmond metadynamics simulations?

During metadynamics calculation, Desmond gives error: -- Allowed momentum exceeded on 2 particles. 7187: force = (-3.0580500e+04 4.4492168e+04 -7.2957642e+03) momentum = (-2.9018593e+01...

03 September 2019 8,099 2 View

Is there a formula to calculate minimum required coverage for NGS given a certain variant allele frequency and sequencing error?

I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...

05 April 2018 9,336 1 View

Any recommendations as to how many bytes/reads/bp per sample are needed for shotgun/16S metagenome sequencing with genus-deep taxonomic profiling ?

20 March 2018 7,619 4 View

How much Resazurin powder should be added to 10 ml of culture medium to prepare a solution for a mammalian cells viability assay?

I have Resazurin powder from Sigma but I don't know which concentration must have a stock solution or a final solution for viability test. Please, help me.

05 December 2017 7,371 2 View

Stat analysis on the sequence of events

Hi all, I need an advise on the correct statistical approach for a sequence of events. 1. I have a data collected from a group of individuals at baseline (BL) and follow-up (FU). 2. There are...

04 June 2017 8,081 5 View

Does culturing of ASC lead to a 100% pure collection of ASC? Or does it contain other adherent cell types?

Dear all, I am starting an experiment with adipose derived stem cells (ASC), but I would like some help or confirmation for the following issue. Background: I am harvesting murine fat from the...

10 July 2016 3,373 17 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View

What is the best antimicrobial agent among metal oxides?

I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?

21 January 2021 7,068 5 View

Does anyone know of a protocol where Sunitinib is directly injected into a tumor in xenograft models?

I'm fairly new to cancer research and there's a host of articles on Sunitinib being administered via oral gavage in mice models but I'm unable to find any information on studies that have explored...

10 January 2021 8,437 3 View