How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

PEI DNA in-vivo transfection concentration?

04 January 2021 6,908 7 View

How to correct a deviation in the molar ratio in an Isothermal Titration Calorimetry equipment?

Our equipment of ITC does not reach the molar ratio (N=1) of a titration of EDTA with Ca2+ in MES buffer pH 6. We have cleaned with DCN 90 and HCl pH 2, but the N value is very low, around...

10 November 2020 3,137 3 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

What would be the recommended color/method for quad-IF?

I'm trying to perform quad-IF staining on frozen tissue sections on a fluorescent widefield (axioplan) microscope. I was wondering what people might suggest for colour/strategy for a co-IF...

13 October 2020 6,863 4 View

How do you remove the tolerance limits when running collinearity statistics in SPSS?

I am using SPSS syntax to determine multicollinearity with predictor variables of interest. When I enter the variables, SPSS is removing some of the variables because the minimum tolerance limit...

02 June 2020 3,639 1 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View

Does anyone knows if is there any open source repository of historical aerial photography that includes the north of Chile?

My PhD includes a remote sensing survey of the Atacama Desert using freely available satellite imagery to detect archaeological sites. So far, I have not found any source of historical aerial...

12 May 2020 1,383 8 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View