I was wondering if there is a reason to use one method over the other. Some literature used acetone and ethanol (3:1), while others used acetone and methanol (1:1). Which mixture do you think leads to better results? And why?
I'm staining for skeletal muscle proteins (e.g. MHCI, COXIV, PLIN proteins ...).
I assume you are talking about fixation for light microscopy and not both light and electron microscopy. If the former, why fix at all? Why not cut frozen sections from unfixed muscle in a cryostat? If a staining procedure requires fixation (many don't), you can do it during the staining procedure and the fixation procedure can be done custom to the staining procedure.
I use fixation on frozen sections for immunofluorescence staining before applying primary and secondary antibodies.
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