Hello! I would very much appreciate some help. I have the 3d structure prediction of subunits alpha and beta of a dioxygenase from Phyre2 and I would like to merge these components in a final model of the complete enzyme using Chimera (preferably) or a similar program. Is this possible? What steps should I follow? Thank you in advance!
Hi,
Are the alpha and beta subunits in two different PDB files and you need a merged two chains in one pdb?
If yes, you can simply do text-editing manually (in your any favourite text editor) then load the pdb in chimera to visualise.
Tanushree Das yes i have two different pdb files. how could i do the text editting in the pdb file.. show some understanding in case of silly question and thank you for your help!
Let you have the PDB file of the alpha subunit, open it with notepad,
1) at the end of chain A, write TER to terminate the chain.
2)then paste the coordinates of the beta subunit (from the source beta subunit pdb file)
3) also paste the connectivity section (CONECT) of beta subunit after the alpha chain CONECT part.
4) END
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