Hi, would appreciate input on what would be the best media to culture primary myofibroblast from the kidneys.
can anyone tell me the grazing incident wide angle spectrometry analysis availability in India??
23 December 2022 9,516 1 View
i knew n values are basically estimated precursor composition for solutions but how to optimize it??
19 December 2022 6,908 2 View
Can anyone guide by providing the procedure to run and interpret Wavelet analysis in Python or R or Matlab? Thanks in advance
09 May 2020 3,933 8 View
I have done 16S rRNA sequence, phenotypic and biochemical characterization, FAME analysis. 16S rRNA sequence exhibited 98% similarity with prauserella corallicola, prauserella sp, prauserella...
28 April 2018 2,720 0 View
Hi, Just wondering if there's any way I can carry out the KSS unit root test on eviews or if there's any other programme anyone knows of that is capable of carrying out the test? Thanks
04 March 2018 9,178 11 View
Hi , I was wondering if anyone has an established protocol on isolation and culture of mouse kidney fibroblasts? or if there is any success in specific isolation using beads or FACS?
18 December 2017 1,298 1 View
Hi, I have been trying to grow primary myofibroblasts from UUO-injured mice kidneys. I used protocol, and grew cells for a duration of 14 days, cells grew well with 80-90% confluency at D14, and...
24 October 2016 5,962 1 View
I have two test shape files (more than 50 points in each file) with slightly varied latitudes and longitudes, but same area (Bay of Bengal). Each file contains Latitude, Longitude and Data_Field...
05 October 2016 8,928 7 View
I am going to attempt using PDGFRB tagged PE antibodies to attempt to isolate these cells from mice kidneys and to culture these cells ( if successful). Would appreciate some advise on this if you...
06 September 2016 9,505 2 View
It is look like a leaf under microscope, but it could be a plankton. Earlier I heard that it was a ciliate, but I didn't get this picture again in my samples. This too found in Bay of Bengal...
04 May 2016 7,557 12 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View