Amirtha Chinnadurai

4 Questions 1 Answers 0 Followers

Questions related from Amirtha Chinnadurai

Protocol for isolation and culture of kidney fibroblasts?

Hi , I was wondering if anyone has an established protocol on isolation and culture of mouse kidney fibroblasts? or if there is any success in specific isolation using beads or FACS?

19 December 2017 1,320 1 View

Media for Primary myofibroblast?

Hi, would appreciate input on what would be the best media to culture primary myofibroblast from the kidneys.

23 January 2017 471 0 View

Poor RNA yield despite high cell confluency seen in the whole kidney/crude lysate culture?

Hi, I have been trying to grow primary myofibroblasts from UUO-injured mice kidneys. I used protocol, and grew cells for a duration of 14 days, cells grew well with 80-90% confluency at D14, and...

25 October 2016 5,982 1 View

Hi, Has anyone isolated myofibroblasts by magnetic cell beads technique?

I am going to attempt using PDGFRB tagged PE antibodies to attempt to isolate these cells from mice kidneys and to culture these cells ( if successful). Would appreciate some advise on this if you...

07 September 2016 9,525 2 View