Mechanism of deprotection of s-acetamidomethyl group using iodine

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Imaging of biomolecules by AFM in liquid using D20 buffer?

Hello Everyone, I am really unable to measure in D2O buffer, for some reason. I even tried with higher forces, like around 250pN ( I usually need 50-80pN when using normal buffer) for my...

10 November 2018 8,924 0 View

Agarose gel electrophoresis?

I am running a 1% agarose gel for separation of the different forms of pBR 322 and I see a band in the well. I do not quite understand the reason why I should see a band which shows almost zero...

09 October 2018 9,005 1 View

Contour length of DNA by AFM ?

I am trying to measure the contour length of plasmid DNA under different conditions. I use AFM to image the DNA molecules and they all seem to be in different topoisomeric states; hence the...

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Plasmid pBR 322 dilution?

I am trying to make dilutions from a commercially available plasmid stock of plasmid pBR 322. The buffer is Tris and EDTA pH 8. After I prepare the buffer, should I autoclave it for it to be DNAse...

05 June 2018 2,591 4 View

I have gold sensors from QSense, how many times can you reuse the same sensor? Are the results reproducible after 1 use?

I usually clean my sensors prior to every measurement but I just wanted to make sure that I am doing the right thing.

02 March 2018 1,151 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Zoltan Dekan

Try this

http://onlinelibrary.wiley.com/doi/10.1002/hlca.19800630418/abstract

Ankita Ray

Dear Zoltan,

Thanks for your help.

Good day to you.

Regards,

Ankita

Cláudia Alves

Hello Ankita!

I'm about to follow this procedure (maybe next week), and I'm curious about the outcome/difficulties you had... could you give me a feedback on this protocol? Maybe some tips?

Thanks in advance!

Cláudia