Mechanism of deprotection of s-acetamidomethyl group using iodine

More Ankita Ray's questions See All

Imaging of biomolecules by AFM in liquid using D20 buffer?

Hello Everyone, I am really unable to measure in D2O buffer, for some reason. I even tried with higher forces, like around 250pN ( I usually need 50-80pN when using normal buffer) for my...

10 November 2018 9,044 0 View

Agarose gel electrophoresis?

I am running a 1% agarose gel for separation of the different forms of pBR 322 and I see a band in the well. I do not quite understand the reason why I should see a band which shows almost zero...

09 October 2018 9,098 1 View

Contour length of DNA by AFM ?

I am trying to measure the contour length of plasmid DNA under different conditions. I use AFM to image the DNA molecules and they all seem to be in different topoisomeric states; hence the...

09 October 2018 8,418 0 View

Plasmid pBR 322 dilution?

I am trying to make dilutions from a commercially available plasmid stock of plasmid pBR 322. The buffer is Tris and EDTA pH 8. After I prepare the buffer, should I autoclave it for it to be DNAse...

05 June 2018 2,679 4 View

I have gold sensors from QSense, how many times can you reuse the same sensor? Are the results reproducible after 1 use?

I usually clean my sensors prior to every measurement but I just wanted to make sure that I am doing the right thing.

02 March 2018 1,254 3 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Zoltan Dekan

Try this

http://onlinelibrary.wiley.com/doi/10.1002/hlca.19800630418/abstract

Ankita Ray

Dear Zoltan,

Thanks for your help.

Good day to you.

Regards,

Ankita

Cláudia Alves

Hello Ankita!

I'm about to follow this procedure (maybe next week), and I'm curious about the outcome/difficulties you had... could you give me a feedback on this protocol? Maybe some tips?

Thanks in advance!

Cláudia