Hello Everyone,
I am really unable to measure in D2O buffer, for some reason. I even tried with higher forces, like around 250pN ( I usually need 50-80pN when using normal buffer) for my measurement but I couldn't track the surface well, there was always a problem with the feedback, it was unstable even with different gains.The tip would retract in less than a minute. I tried different engage forces but nothing really seemed to make it better. I cannot seem to put a finger on the reason. I never had this problem before.
Do you think this could be because of the difference in densities of water and heavy water ?