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Questions related from Ankita Ray
Hello Everyone, I am really unable to measure in D2O buffer, for some reason. I even tried with higher forces, like around 250pN ( I usually need 50-80pN when using normal buffer) for my...
11 November 2018 9,006 0 View
I am trying to measure the contour length of plasmid DNA under different conditions. I use AFM to image the DNA molecules and they all seem to be in different topoisomeric states; hence the...
10 October 2018 8,388 0 View
I am running a 1% agarose gel for separation of the different forms of pBR 322 and I see a band in the well. I do not quite understand the reason why I should see a band which shows almost zero...
10 October 2018 9,062 1 View
I am trying to make dilutions from a commercially available plasmid stock of plasmid pBR 322. The buffer is Tris and EDTA pH 8. After I prepare the buffer, should I autoclave it for it to be DNAse...
06 June 2018 2,644 4 View
I usually clean my sensors prior to every measurement but I just wanted to make sure that I am doing the right thing.
03 March 2018 1,212 3 View
Can anyone help me by citing an appropriate literature for the mechanism of deprotection of s-acetamidomethyl using iodine?
07 July 2017 7,473 3 View