Hi Guys,
Has anyone tried measuring contractility in hiPSC derived cardiomyocytes? If yes, what method and machine is required to measure the contractility vs time?
Any leads would be very much helpful.
Thanks in advance
By using mobile phone, grab the video. Then, digitized video can be dynamically analyzed.
Here is an article where we measured the contractility of valve interstitial cells.
Article Valve interstitial cell shape modulates cell contractility i...
To summarize the protocol, we create patterns by employing soft lithography techniques and measure the contractility using videos captured on a stereomicroscope. We used custom-MATLAB codes to measure the stress but the reference provided has all the relevant equations used. The thin films were created using PDMS elastomers but based on the availability of resources, you can also create gelatin thin films.
Very easy to measure with this method，see paper“MUSCLEMOTION: A Versatile Open Software Tool to Quantify Cardiomyocyte and Cardiac Muscle Contraction In Vitro and In Vivo” fulltext link:https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.117.312067?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
Prashanth Ravishankar Thank you for the article. I will try this method too.
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