Paul Milham
Please give a bit more detail?
Do you want to measure photosynthates? Sugars and starch? I presume TLC is thin layer chromatography.
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Abhijit Mitra
Sample Collection: Collect samples of water (or another medium) at various times to measure changes in TIC. The sampling frequency will depend on the expected rate of change in CO2 uptake. TIC Measurement: Measure the TIC in each sample. TIC represents all forms of inorganic carbon in the system, including dissolved CO2, carbonic acid, bicarbonate, and carbonate ions. Methods for measuring TIC typically involve acidifying the sample to convert all forms of inorganic carbon to CO2, which is then quantified using infrared gas analyzers or other carbon detection methods. Control Measurements: Alongside your experimental measurements, take control measurements to account for any changes in TIC that are not related to biological activity (e.g., physical absorption/desorption of CO2). Calculate CO2 Uptake: Analyze the changes in TIC over time. The decrease in TIC in your samples, compared to control samples, can be attributed to biological CO2 uptake. This is based on the assumption that any process consuming CO2 (like photosynthesis) will reduce the TIC. Correction for Other Processes: Account for any other processes that might affect TIC, such as calcification or dissolution, which can also change the carbonate chemistry.
To measure CO2 uptake when you have Total Inorganic Carbon (TIC) data, you can follow a series of steps that involve calculating the change in TIC in a system over time. This method is commonly used in aquatic environments where CO2 is absorbed from the atmosphere or released by organisms. Here’s how you can approach it:
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