Hello everyone, I posted this before but I am still looking for improvements in my method to detec hydrogen sulfide in plasma from rats.
I am currently using the methylene blue method, however, I can not see any reaction in my tubes. I will try to use degassed water in an experiment today, but any tip or advice would be really welcome. Moreover, I found some dicrepancies regardin the molarity of the acid soluction to dissolve FeCl3 and N,N-dimethyl-p-phenylenediamine sulfate solutions. Some papers described they used 1.2 and 7.2 milimolar (mM), respectively and others described 1.2 and 7.2 molar (M), respectively.
Any kind of advice is welcomed, thank you
Molar (M) as I remember dissolving the perchloric acid from the bottle, whose molarity is approx 12M, to obtain the working solutions.
Did you do a calibration curve? If so, did you see any reaction in those tubes?
Otherwise it could be that hte levels in plasma are very low. Did you check bibliography about what would be the expected values?
Hi
I am following that protocol:
Xinggui Shen,a Christopher B. Pattillo,a Sibile Pardue,a Shyamal C. Bir,a Rui Wang,b and Christopher G. Kevila,
Prepare the following solutions.
Standard curve is working well.. made by NaHS..5-400uM
Problem is sensitivity at this moment.
Good luck.
Thank you Turtushikh Damba ,
I would like to ask you just one thing: how do you obtain degassed water?
That's still big issue for me. I normally just filter it by 0.2um filter.
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