Hi All,
Please here are some other pictures that I got. May somebody help me with the name of this fungus? I am working on water hyacinth and these pictures come from damage leaves by pathogens
Thanks
Dear Sonia is may be possible Rhizoctonia sp. The hyphae of Rhizoctonia have the following characteristics: 1) some shade of brown; 2) a special type of cross wall within the hyphae, called a dolipore septum; 3) each cell is multinucleate (has many nuclei) rather than binucleate; 4) branches that are produced at right angles; 5) no asexual spores are formed by the mycelium. You can isolate DNA of your culture and test some primer for identification.
Thanks to you all for your detailed answer. I have several pictures like that which are really difficult for me to identify.
Thanks once more
I agree that this looks like it could be a Rhizoctonia species, however, it would be useful to know what medium was used. Morphological characters can vary depending on the type of medium used.
For characteristics of the genus Rhizoctonia, another important one is lacking clamp connections. Also it should be noted that not all Rhizoctonia species are multinucleate. See for example Sneh et al. 1991, Identification of Rhizoctonia Species (APS Press), for other species and information on how to identify them. Note the dolipore septa in hyphae that lack clamp connections is an important characteristic to identify Rhizoctonia and is visible with a light microscope at higher magnification than the images presented (e.g Moore 1987. Mycotaxon 29:91-99). If these characters are present, young hyphae can be stained to examine the nuclear state for species identification.
Dear Sonia, you can find an interesting article about water hyacinth diseases on the Researhgate.
Regards, Franci
Article Biological Control of Water Hyacinth by Using Pathogens: Opp...
Thanks you all, I am very glad and will have go through all your suggestions. This research area is new to me, and I am still learning. Thanks once more
Agreed with all.It may be Rhizoctonia or Sclerotium . Sclerotia could be seen in slide 2 and 3
It is a Rhizoctonia sp. You need to determine number of nuclei by cell. R.solani is multinucleated. The culture is old because it is forming sclerotia
It is Rhizoctonia culture with sclerotia production. Mycelia are Perpendicular and no sporulation but production resistance form is present.
imperfect form of Rhizoctonia sp. fungus. You may determine type of anastomosis group of mycelial cells for species identification.
Good luck
Hi Kenfackvoukeng: It is look like Macrophomina spp, this is not Rhizoctonia.
First, you need confirm that is R. solani and later to do anastomosis tests. Can use stain made with lactic acid and fucsin acid 0.25% and apply it to colonies produced on thin film of water agar . 6.5 ml per plate
Dear, Kenfackvoukeng Sonia Nadege
Very good after noon, This is Macrophomina phseolina = Rhizoctonia bataticola and also macro sclerotia forming in adverse condition and cuture.
Thanks you to you all for your help. I am really grateful
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