Hi Asif,

I am not much familiar with GWAS studies and how people support their data after doing GWAS. But you need to support your finding with more information. Usually, it's not really scientific to relate genes to certain functions only based on one or two experimental data. I assume you have a couple of options to prove your theory for that locus being related to the flowering time:

1) isolate that gene, make mutant form or over expression transgenic lines of your gene, compare to the wild type and see if they show any difference in flowering time with the WT.

2) if you don't have enough time for going all the way for making transgenic lines, try to backup your theory with bioinformatics, search for the possible relations of your gene with the flowering pathway genes (SPLs, FT, FUL, AP...)

you should also read some published papers and see how they have supported their GWAS data further to get an idea. otherwise, you can only mention that ''this locus MIGHT be playing some role in the flowering time''.

Hope you find it helpful :)

Dear Asif, You can suppose that your gene Os10g30380 may influence the flowering time as its homolog in Arabidopsis influence FT. But its just an assumption but not confirmation. You should do the complementation test if possible as suggested by Shirin Mirlohi.