Syed Adeel Zafar

8 Questions 13 Answers 0 Followers

Questions related from Syed Adeel Zafar

Why I am getting yeast colonies with empty AD on DDO?

For my Y2H experiment, my yeast gold strain transformed with an empty AD vector (PGADT7) is growing on DDO medium (not growing with empty BD). What could be the possible reasons and how to figure...

20 October 2022 2,332 1 View

I purchased electro-competent cells of Agrobacterium strain GV3101 from a company. Can I use these competent cells to make new cells?

I purchased electro-competent cells of Agrobacterium tumefician strain GV3101 from GoldBio company. Can I use these competent cells to make new competent cells or not? If yes, do I need any...

01 June 2021 8,695 4 View

A leaf yellowing and defective chloroplast mutant showed increased expression of all 7 genes involved in photosynthesis pathway (KEGG) compared to WT?

Biologists please help me get out of confusion. A leaf yellowing and defective chloroplast mutant showed increased exp of all 7 genes involved in photosynthesis pathway (KEGG) compared to WT...

12 April 2020 9,654 0 View

How can I be successful in making a vector construct with very large insert size of 10 kb?

Dear fellows, I am trying to insert a gene of almost 10 Kb into pCAMBIA 1305.1 plasmid (12.8 Kb) digested with respected enzymes. I have divided the gene into two fragments (S1 and S2) of 5 kb...

26 December 2016 8,828 1 View

Standard curve for Hydrogen peroxide but the solution looks transparent?

Dear fellows, I want to make standard curve for H2O2 using different concentrations of H2O2 but the values are same as blank or very near to it when I observed the absorbance at 560 nm. One thing...

02 November 2016 367 3 View

Why I could not amplify a 2.5 Kb promoter region??

I am trying to amplify the promoter region of 2.5 Kb of a rice gene, it is highly GC rich, but I could not amplify it even after changing primers. My primer is totally 43 bp including the 15 bp...

22 August 2016 1,306 3 View

Measurement of Enzymatic antioxidants?

For the measurement of enzymatic antioxidants like SOD, POD, CAT, APX and Total soluble protein, how to convert the absorbance reading (OD) into ug/ mg protein?

22 June 2016 1,350 5 View

I have a question that during primer designing I got different length of forward and reverse primer of same gene. Can I use this primer for RT-PCR?

25 May 2015 6,784 9 View