I am trying to fix MDA-MB-231 cells for confocal microscopy. The cells are seeded in PEG based scaffold using collagen based cell seeding matrix that acts as ECM. I will be imaging in hopes to find the shape of cell spheroid formed in the scaffold.
Number of cells seeded - 2 to 2.5 million
I need the fixed cells to survive transportation since I will be travelling to a different facility for confocal imaging. I need them to survive or retain morphology after fixing for atleast 24 hours.
Hi Kavil, You can use 4% Paraformaldehyde to fix the cells on scaffolds.
Hi Dr. Subia Bano I am using ultra-low attachment plates (Corning), I want to collect my spheres and want to fix them onto a glass slide for further ICC. Could you pl guide me for the same. Thanks in advance.
Best,
MJ
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