There is a band even though I never load any sample into the well. Is it because of my PCR technique or gel preparation skill or any other?
Can someone help me with this?
Much appreciate! Thanks!
It may be due to contamination from running buffer and tank. Wash the tank and use fresh TAE buffer to get rid of this issue.
best
From what i can understand, you load pcr product into the gel, but you get bands at the top. It seems like you put too much dna sample in your pcr reaction, try to put less
It may be due to contamination from running buffer and tank. Wash the tank and use fresh TAE buffer to get rid of this issue.
best
Besides using fresh TAE buffer in tank, prepare gel from fresh TAE as well.
Dear Wong,
I agree with Subagar and Rezwana try to prepare fresh TAX buffer.Then, redo the gel with the fresh buffer and also clean the tank and add new fresh buffer.
How come you have two bands for some smaples? For example lanes 4, 5 and 9 ?
Anyway, from the aspect of the marker I can say that you have some problem with the gel the bands are not sharp how they should be...
Again, do all fresh preparations for gel and buffer to run. Use pipette tips with filter and pay attention at any contamination. If you do not load any sample you should not have any band in gel...
Do you have in this gel any result of your PCR or you only loaded the marker ?
How are your positive and negative controls in PCR? Any bands?
Thanks all for the sharing.
Stefania Mang , I'm running the gel with the sample. Column 4 & 5 are the bands and Column 9 was the positive control and Column 10 is negative control.
Probably the fresh buffer may solve my problem. Thanks once again.
Since that band is appearing in the same column as your DNA ladder, it's something from the gel pouring and/or running equipment and NOT from your PCR. As others have said, get fresh buffer, wash your equipment, and try again.
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