From what i can understand, you load pcr product into the gel, but you get bands at the top. It seems like you put too much dna sample in your pcr reaction, try to put less
I agree with Subagar and Rezwana try to prepare fresh TAX buffer.Then, redo the gel with the fresh buffer and also clean the tank and add new fresh buffer.
How come you have two bands for some smaples? For example lanes 4, 5 and 9 ?
Anyway, from the aspect of the marker I can say that you have some problem with the gel the bands are not sharp how they should be...
Again, do all fresh preparations for gel and buffer to run. Use pipette tips with filter and pay attention at any contamination. If you do not load any sample you should not have any band in gel...
Do you have in this gel any result of your PCR or you only loaded the marker ?
How are your positive and negative controls in PCR? Any bands?
Stefania Mang , I'm running the gel with the sample. Column 4 & 5 are the bands and Column 9 was the positive control and Column 10 is negative control.
Probably the fresh buffer may solve my problem. Thanks once again.