I have a gene that I need synthesized that is about 4150 bp long. Getting it synthesized completely in one piece will be expensive and time-consuming. I've used overlap PCR before to make ~1000 bp products, but I'm wondering if I can instead use overlap PCR to connect the two halves of my gene? (~2 pieces each at 2000 bp for ~4000 bp total) . Synthesizing fragments ~2000 bp is much cheaper and faster.

Thanks in advance!

Andrew

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