Andrew Michael Hogan

5 Questions 30 Answers 0 Followers

Questions related from Andrew Michael Hogan

Trimethoprim selection for Pseudomonas aeruginosa?

Hi everyone, I'd like to transfer an integrative plasmid to P. aeruginosa PAO1 and the resistance marker is trimethoprim. Has anyone used that selection before in PAO1? I can't find much...

02 February 2020 4,885 1 View

Does formaldehyde fixation affect bacterial cell morphology/nucleoid condensation?

We work with a difficult-to-stain Gram-negative bacterium. We need to stain the membrane and nucleoid with different dyes (unfortunately, we cant use the FM series of membrane dyes from...

04 April 2019 9,240 2 View

Maximum size for Overlap extension PCR (SOEing PCR)?

I have a gene that I need synthesized that is about 4150 bp long. Getting it synthesized completely in one piece will be expensive and time-consuming. I've used overlap PCR before to make ~1000 bp...

11 November 2017 5,377 4 View

Is it possible to use DAPI and PI simultaneously?

I'd like to stain bacterial cells to see all of the cells' nucleiods along with which cells have disrupted membranes. Can DAPI and PI be used in the same cells? I know their emissions are very...

01 January 1970 1,787 4 View

Does anyone have a protocol for staining gram negative bacteria with the membrane stain DiI-C18?

Currently, I stain with 1-5 ug/mL DiI-C18 for 30 mins at 37oC, but most of the cells aren't staining. I can't fix my cells, because my cells express GFP and I've noticed decreased GFP signal after...

01 January 1970 9,423 1 View