Maximum dilution of DNA?

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Mostafa Haghpanah

hi, dear Kamesh,

This is dependent on your study and amount DNA extracted.

However, I usually dilute DNA in 100 microlites TE and sometimes I have to dilute more.

António Maximiano Fernandes

It depends the amount of material you extract. For tissue cells 100 uL is ok. But if the material you extract is few you should add less amount

Kamesh Krishnamoorthy

Thanks for your valuable answers