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Questions related from Pavel Nesmiyanov
I'm using venous blood drawn into sodium citrate, then lyse RBCs with ACK lysis buffer. The amount of blood is usually around 100 uL. Doing 4-5 hours stimulation with PMA-i, adding BfA 1 hour post...
12 May 2025 5,942 0 View
I'm running plaque assay with 10 serial 10-fold dilutions of VSV viral stock that I've harvested a while ago. It's been 18 hours now after infection and agar overlay. The cells in all 10 wells (I...
04 December 2024 3,663 0 View
To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify....
24 January 2024 6,563 2 View
Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...
06 September 2023 313 1 View
Some claim that certain foods, including citrus for example, directly activate mast cells (like some drugs do in IgE-independent way) and thus induce histamine-mediated symptoms, being so called...
26 April 2021 9,549 2 View
I need to prepare a bacterial lysate of mycobacterium where liquid culture contains 10^8-10^9 cells per mL. To increase yield I sonicate 15 mL at once. I tried different regimens of sonication,...
10 March 2021 680 6 View
Is there a possibility that a virus (RNA or DNA) can be eliminated from a cell while the infected cell stays alive? Are there any mechanisms except apoptosis/pyroptosis/necrosis etc?
01 January 1970 7,184 4 View
Why do people use term "APC", or, say "aAPC" for artificial cells that are employed for CAR T cells study and production? Said cells carry the necessary antigens for T cell stimulation, but these...
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