Does anyone know for a commercially available defined media for S.aureus?

I would like to find an alternative to TSB, with known composition. Thank you so much!

10 November 2017 9,801 2 View

Way to oxidize flavins and related molecules?

I would like to oxidize flavins and if possible also quinones in bacterial supernatant. Is there an enzyme one could use for that? Or any other "oxidant"? I use M9 minimal medium. Thank you so...

10 November 2017 342 3 View

Can one fix bacteria with isopropyl alcohol?

I would like to fix S.aureus on plastic surfaces and I cannot use EtOH or acetone since they dissolve my plastics and I would just like to try another method then formaldehyde. Than you so...

09 October 2017 4,577 3 View

Sequential spin coating of the same material to increase film thickness?

I am working with PEDOT:PSS thin films that I routinely produce by spin coating on play microscope slides. For various measurements I would need to have films of different...

06 July 2017 301 9 View

Is there a transposon library of S.aureus 8325-4 one can obtain?

I am looking for transposon library in S.aureus 8325-4 strain.

06 July 2017 2,565 6 View

Can bacteria be cryofixed?

I am using a conformational dye that bonds to some structures on bacterial membranes and formaldehyde fixation does not really work for me. Thanks!

04 May 2017 4,458 5 View

NADH fluorescence in filtrates of bacterial cultures increases upon heating??

Upon filtering (0.2um) or ultrafiltering (3kDa) an exponential culture of Salmonella enteritidis in M9 I can measure fluorescence at the wavelengths expected for NADH. However, if I heat these...

10 November 2016 1,851 5 View

Why am I unable to blot a recombinant protein from mouse liver ?

We have used hydrodynamic injection (http://www.mjhid.org/article/view/418/620) to deliver DNA to liver where we have shown with microscopy and IHC that the protein is present. We have also used...

01 February 2015 5,041 12 View

Can anyone suggest other tags for purifying proteins under denaturing conditions other than Histidine-Tagged?

We would like to find an option to purify proteins under denaturing conditions (= 6M guanidinium hydrochloride) that is not his column (or similar, cobalt etc). Any ideas? Thanks a lot!

01 February 2015 3,486 10 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View