10 Questions 23 Answers 0 Followers
Questions related from Karen Butina
I would like to oxidize flavins and if possible also quinones in bacterial supernatant. Is there an enzyme one could use for that? Or any other "oxidant"? I use M9 minimal medium. Thank you so...
11 November 2017 400 3 View
I would like to find an alternative to TSB, with known composition. Thank you so much!
11 November 2017 9,872 2 View
I would like to fix S.aureus on plastic surfaces and I cannot use EtOH or acetone since they dissolve my plastics and I would just like to try another method then formaldehyde. Than you so...
10 October 2017 4,640 3 View
I have spin coated films of PEDOT:PSS on glass (and gold) and would like to map their conductivity The films are about 150nm thick. I have tried using PeakForce TUNA (Dimension Icon, Bruker) but...
09 September 2017 6,636 2 View
I am looking for transposon library in S.aureus 8325-4 strain.
07 July 2017 2,618 6 View
I am working with PEDOT:PSS thin films that I routinely produce by spin coating on play microscope slides. For various measurements I would need to have films of different...
07 July 2017 368 9 View
I am using a conformational dye that bonds to some structures on bacterial membranes and formaldehyde fixation does not really work for me. Thanks!
05 May 2017 4,518 5 View
Upon filtering (0.2um) or ultrafiltering (3kDa) an exponential culture of Salmonella enteritidis in M9 I can measure fluorescence at the wavelengths expected for NADH. However, if I heat these...
11 November 2016 1,987 5 View
We have used hydrodynamic injection (http://www.mjhid.org/article/view/418/620) to deliver DNA to liver where we have shown with microscopy and IHC that the protein is present. We have also used...
02 February 2015 5,115 12 View
We would like to find an option to purify proteins under denaturing conditions (= 6M guanidinium hydrochloride) that is not his column (or similar, cobalt etc). Any ideas? Thanks a lot!
02 February 2015 3,542 10 View
