When doing a systematic review do you rely on both published and unpublished material?

What is your experience in doing a systematic review existing literature? Do you rely on only published material, or can unpublished material add information? Can PBE material be used to...

01 February 2018 6,959 1 View

What do you think of "open access" journals. Would you publish in one of them?

Doing literature searches is difficult enough. In this era of on-line journals it is sometimes hard to access information. One way might be to search the "open access" or "free" journals. What...

31 December 2017 9,903 8 View

What statistical analysis procedure should I use?

We are completing a study of second grade children's responses to standardized testing (Common Core requirement) done three times a year (Fall, Winter, Spring). We have 50 children enrolled, 17...

10 November 2017 3,795 0 View

Are surveys a valid way to measure perceptions of professional development attendees?

We use surveys in our research, but wonder what others think of their validity.

02 March 2017 5,755 2 View

What theory(ies) of speech perception is (are) being taught in undergraduate speech and hearing science classes?

I am teaching a class in May, and wonder what is being taught elsewhere. Jeff Knox St. Ambrose University

02 March 2017 7,603 4 View

What reading program did your teachers follow?

01 January 1970 4,341 7 View

Observing gesture use in elementary teachers?

01 January 1970 5,903 11 View

What techniques do you use to improve the vocal output of Parkinson's patients?

01 January 1970 4,352 0 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which period is considered as study period in research?

Is that starting from proposal development to result writeup or just from data collection to result writeup and reporting (publishing)?

28 February 2021 910 2 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View