VDR gene in Echinococcus granulosus ?

In recent project we would like to evaluate the expression level of VDR gene in Echinococcus granulosus . For primer design we need that primer and the accession number of mRNA for this...

21 February 2021 8,673 1 View

How can extract RNA from plasma by trizol?

Hi Dear Colleagues, Could anyone explain an optimized protocol to extract total RNA from plasma samples? We would like to extract total RNA from plasma samples through Trizol, however, after the...

11 September 2020 7,456 3 View

Does anybody know any other visualization tool such as YellowBrick for Machine Learning visualization in Python?

I need a visualization tool similar to YellowBrick for the visualization of dataset and Machine Learning results in Python.

07 January 2019 2,503 2 View

What experiment type to choose for analysing TaqMan genotyping experiments with TaqMan Genotyper software?

Hi, I have assessed 4 SNPs by TaqMan genotyping assays from Applied Biosystems using StepOne plus realtime PCR system. Now, I tend to analyze my results via TaqMan genotyper software V 1.0.1....

17 September 2018 9,660 5 View

Fitting DFT calculated parameters in an EAM/alloy format?

Dear all, I have calculated necessary parameters including elastic constants, specific heat, equilibrium lattice constants, cohesive energies, migration and formation energies of defects and ......

21 November 2016 8,601 5 View

Zr-H and Zr-He eam/fs potential file for LAMMPS?

Greetings, A quick guide to access Zr-H and Zr-He (hydrogen and helium diffusion in Zr) eam/fs potentials suitable for LAMMPS will be appreciated. Regards Afshin

27 June 2016 7,872 10 View

DFT calculations of defect properties using Quantum Espresso?

Deal all, I want to calculate defect properties (i.e. formation energies and migration barriers, ...) in vanadium using DFT- Quantum Espresso, and I am wondering which of the available...

18 May 2016 9,850 3 View

Why does the Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody give me an additional band on Western Blots?

20 September 2015 9,842 2 View

What are the best methods for the detection of macroautophagy?

23 December 2014 10,019 5 View

What is the mechanism of Heavy metals adsorption by Al2O3 nano particles?

13 October 2014 7,916 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View