Hello!

Currently I'm trying to do microrheology by means of Fluorescence Confocal Microscopy in order to compare these results with the macrorehology data. Unfortunately, my microrheology results don't match with the macrorh. data.

My sample is very viscous hydrogel consisting of PEO and PMAA. Furthermore, I'm using 40 nm yellowish FluoroSpheres in order to track the particles in my sample and to calculate G' as well as G'' then. G' and G'' from microrehology experiments always have lower values than the macrorh. data so that they don't match at all.

My technical parameters: T = 25°C; FluoroSpheres Size: 40 nm; FluoroSpheres Conc: 1 nM, Experiment Duration: 4h; confocal volume: 5

I already varied the particle size (100 nm, 20 nm) and conc. of the particles (3 nm, 1 nm and 0.1 nm)

Do you have any ideas which parameters are also necessary?

Thanks in advance.