M9 minimal media reliable recipe?

25 November 2024 0 9K Report

Hello, i am cultivating E. coli transformant in M9 minimal media for heterologous expression. I am having trouble with growth so i need someone who has experience to give me recipe for M9 minimal media, because i tried with 2 recipes and it did not gave good results. Also, i am using N15 and C13 labeled ammonium-chloride and glucose, respectively.

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