Can you provide RT-PCR raw data to train our machine learning algorithm?

Dear colleagues working with RT-PCR devices, We are a team of data scientists, biologists, web developers working on an open-source machine-learning algorithm to contribute suppressing the COVID19...

06 May 2020 1,789 3 View

Is there a procedure that I can use to remove the N-terminal acetylation on a peptide and increase the solubility?

I am working with a series of 13-mer peptides that are quite hydrophobic. Most of the peptides do not contain any acidic or basic residues. The peptides are capped at the ends (N-terminal...

06 July 2016 6,756 3 View

Hi! Question about Hierarchical regression to test interactions.

Hi! I used Hierarchical regression to test interactions - step 1: main effects step 2: three 2 way interactions step 3 one 3-way interaction The R-square difference between step 2 and step 1 was...

11 January 2016 9,589 8 View

Is this true that 6M GdnHCl will degrade my peptide if I try to purify the peptide using 6M GdnHCl to dissolve it?

The peptide does not contain any acidic or basic residues and it is capped on the ends. We tried to dissolve the peptide in several organic solvents. It dissolved in some of the solvents, but...

25 June 2015 9,665 3 View

Importance of ethics in education?

What is your opinion on the issue of ethics? Do you emphasize its importance to your students, or you believe they had a chance to get familiar with ethics at previous modes of study(primary and...

01 January 1970 633 11 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View