Lazar Gardijan

7 Questions 4 Answers 0 Followers

Questions related from Lazar Gardijan

Staphylococcus aureus stringy growth in FBS?

I am doing virulence gene expression analysis of Staphylococcus aureus in LB medium and FBS. The overnight culture in LB was as usual, but in the FBS there was only like yarn with strings. I...

28 February 2025 8,747 0 View

M9 minimal media reliable recipe?

Hello, i am cultivating E. coli transformant in M9 minimal media for heterologous expression. I am having trouble with growth so i need someone who has experience to give me recipe for M9 minimal...

25 November 2024 8,704 0 View

E. coli cell free expression systems - yield?

I have a peptide that i cant express in E. coli cells, so i wanted to try with cell free expression kits. I want to know what yield can i expect and since the idea is to use my peptide for NMR,...

22 August 2024 1,302 1 View

Do these colonies look like E. coli?

I am cloning in BL21 and ive got these colonies. Before the weekend the big colonies were different and smoother edges. But after 2 days in +4⁰C they look like in pictures. I thought the smaller...

26 February 2024 5,473 0 View

Bacteriocin expression in pET-19b?

I am looking for vectors for expression of bacteriocin, and from pET series I think that 19b fits the most, I am just having few questions. When I clone my insert in NdeI and XhoI, i will have...

13 December 2023 2,364 5 View

Cell-free expression experience?

I am having trouble with expression of 6 kDa long bacteriocin in pQE and pMAL systems. So i wanted to try the cell-free expression and i want to hear experiences with cell-free expression systems...

14 July 2023 3,164 3 View

Assays for determining antimicrobial nature?

I am working with an unknown antimicrobial agent from soil bacteria. On well diffusion assay it showed antimicrobial activity on few pathogens, but the widest and clearest zone was on S. aureus...

17 September 2021 6,706 3 View