Lazar Gardijan

4 Questions 4 Answers 0 Followers

Questions related from Lazar Gardijan

Do these colonies look like E. coli?

I am cloning in BL21 and ive got these colonies. Before the weekend the big colonies were different and smoother edges. But after 2 days in +4⁰C they look like in pictures. I thought the smaller...

26 February 2024 5,316 0 View

Bacteriocin expression in pET-19b?

I am looking for vectors for expression of bacteriocin, and from pET series I think that 19b fits the most, I am just having few questions. When I clone my insert in NdeI and XhoI, i will have...

13 December 2023 2,283 5 View

Cell-free expression experience?

I am having trouble with expression of 6 kDa long bacteriocin in pQE and pMAL systems. So i wanted to try the cell-free expression and i want to hear experiences with cell-free expression systems...

14 July 2023 3,097 3 View

Assays for determining antimicrobial nature?

I am working with an unknown antimicrobial agent from soil bacteria. On well diffusion assay it showed antimicrobial activity on few pathogens, but the widest and clearest zone was on S. aureus...

17 September 2021 6,667 3 View