Some of my analytes have values like 1, 0, and 23, while others have less than
Thank you, Lyudmila for your valuable suggestion.
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
Hi All, Our Luminex 200 sat unused for a few years. After many sanitise/wash/alcohol flush cycles, and repeated stringent cleaning cycles, CAL2 always fails (The acquisition ran to sample empty)....
10 March 2024 4,055 0 View
Dear colleagues, We are planning to use Human XL Cytokine Base kit for evaluation of concentration of several cytokines. The plan was to measure concentration of cytokines both in blood serum, and...
20 February 2024 7,692 0 View
I have recently coupled some magplex beads to protein, using the biorad kit and beads following a standard protocol from my supervisor that has worked well previously. When I went to count these...
06 July 2023 1,794 1 View
I am losing beads in almost every well of 96 wells plate and also my beads per second is very low like highest was only 5. What could be the possible cause. I take appropriate time between the...
17 June 2023 1,168 2 View
I'm planning to process mouse inguinal and popliteal lymph nodes for Luminex assay. Does anyone have experience with it? What reagents to use? Right volume and dilution of the sample etc?
01 March 2023 4,663 0 View
I am looking for a commercially available GPL core antigen to use in a Luminex immunoassay. I am aware of the Capilia MAC Ab ELISA kit, but I am searching for just the antigen to couple to Luminex...
01 March 2023 9,433 0 View
Dear all, I intend to study T helper cells cytokines in mice pancreas tissue, please recommend a suitable buffer solution for homogenization step! In the protocol, they mentioned "For...
20 August 2022 5,065 0 View
What should be used as alternative cleaning fluid for luminex flow cytometer. I know that we can use contard 70, but not sure what percent, 10% or 15 % ? Do they have bleach in it with Luminex...
18 July 2022 1,427 1 View
We have compared a multiplexed luminex assay with the standard singleplex assays for ferritin, sTFR and RBP. The luminex values are manifold higher. We have used standards and purified proteins...
15 July 2022 5,265 0 View