I want to measure inflammatory cytokine in urine sample. Bio Rad and affymetrix had kit for Urine Toxicity but it’s limited to only few markers that I am not interested. I am wondering whether the kit made for serum/ cell-culture supernatant that measures about 40 cytokine will it be suitable for measuring cytokine in urine?
Kit such as: Bio-Plex Pro Human Cytokine 27-plex Assay #M50-0KCAF0Y
High salt concentration and difference in pH that can be managed by diluting urine with buffer. But I am worried that the proteins in urine are already denatured and it will not work with the kit that it made for serum.
Some companies have arrays that measure about 500 different factors all at once and can be used for everything.
Marcia Moss ... Thanks for your reply but what kind of assay are they? and if can name the compaines, it will be realy helpful.
The company is Ray Biotech. They biotinylate all the proteins in a sample, and then they have an antibody array with 500 different antibodies. They capture the biotinylated proteins, wash the slide, and then look for a signal for each well. They can do sera, plasma, urines etc. Just look them up.
Thanks,
Marcia
- Badges
- Science topic
- Similar topics
- Correction