Low signal - Circadian luciferase assay in cell culture?

11 November 2018 1 4K Report

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear bottom plate. We get good cycling, but our overall lumen counts seem low (

Kristina M Cook

Hi Jackie,

Sorry I can't answer your question but we are trying to set up a similar assay with a TECAN 200 as well. How long are you reading the wells for? What kind of settings do you use for the TECAN? Any tips would be greatly appreciated.

I saw a paper somewhere that used something like 30min reads from memory.

Also, have you tried white plates? They can help enhance the signal from other luciferase assays that I have run.

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