Jacqueline Lane

6 Questions 11 Answers 0 Followers

Questions related from Jacqueline Lane

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

11 November 2018 3,557 1 View

Can anyone recommend a company to order lentiviral particles or target plasmid from?

We have a plasmid of interest and we would like it cloned into a lentiviral plasmid followed by production of particles for infection in mammalian cells. Any company/core facility recommendations...

02 February 2015 8,980 2 View

Power calculations for genetic risk score

How do you approach power calculations for genetic risk scores?

10 October 2013 1,885 1 View

What is your preferred maxi prep kit for plasmid DNA grown in bacteria?

We currently use The HiSpeed Qiagen Maxi Prep kit. We are shopping around for other options. Does anyone have a preferred method/kit? How do yields compare? ease of method? cost? thanks!

02 February 2013 7,021 8 View

Does anyone know how to calculate regression dilution in a multivariate regression model?

We have an association between a dependent and independent variable adjusted for 3 covariates. Our dependent variable is measured via self-report, which has a .47 correlation to objective...

01 January 2013 5,203 0 View

How can I reduce the viscosity of extracted genomic DNA?

I recently extracted DNA from iPS cells using cell lysis buffer + prot K followed by ethanol precipitation. The resulting genomic DNA is viscous and hard to pipette. Any thoughts on how to...

01 January 2013 8,048 13 View