I am using pET29 for cloning. Digesting with kpnI and NdeI, the result was very good looking on the gel; i did gel purification but i dont see a trace of band after purification!
Since i nèed to use the final product for protein expression, i was wondwring if i could skip the gel purification step and do ligation wthout gel purification?
I am attaching the pic of my digestion before gel purification.
Best