Loss of GFP Signal After Loading DiSBAC2(3) for Voltage Measurement?

01 January 1970 0 2K Report

Hello,

I am interested in measuring voltage changes in my cells under high and low potassium concentrations. My cells express GFP, and after taking images under low or high potassium conditions, I loaded the dye DiSBAC2(3). However, I lost all GFP-positive signals.

Why did this happen? I tried using the lowest concentration of the dye, between 1 and 5 µM.

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