Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

Could you recommend a book on White Mice brain or on pathology of white mice (Mus musculus) brain?

The book will be used as a reference.

17 March 2018 739 3 View

Does anyone know a replacement for RNA carrier for Qiamp minelute virus spin kit?

I am intended to sequence (via Illumina) viral nucleic acids, including RNA. I was informed that using RNA carrier is not recommended in this case. However, since my samples will have a low...

29 November 2017 8,244 2 View

LPS dosage with LAL QCL-1000 Chromogenic endpoint Lonza, does anyone with experience?

I'll dosage LPS with LAL QCL-1000 Chromogenic endpoint Lonza, and i have many questions about this. Has anyone used this kit yet? Does anyone know if all materials should be depyrogenised? Can the...

03 April 2017 7,719 1 View

Dear colleagues, Can anybody help me identifying these fish vertebrae?

25 May 2016 2,532 1 View

I am starting a research on schools and clubs offering sports. Anyone know any database on the subject?

07 March 2016 9,875 3 View

How can I do PET Spheres/aggregate for concrete?

My question is about some heat treatment for RE-PET from bottles to obtain particles to use as aggregate in concrete.

19 January 2015 7,616 2 View

Where can I find software for data classification using label propagation?

Dear colleagues, I am doing some research in data classification, and I would like to compare to some classification approaches that uses label propagation (see e.g. Zhu, Ghahramani,...

22 October 2014 3,161 3 View

Have you ever had problems with 1.8 K close cycle refrigerator (Joule Thomson valve cold head)?

We are having problems with our JT cryostat. We have cooled down our system three or four times and it easily reached the base temperature (~ 1.8 K). However we cannot cool down the system below...

15 July 2014 8,288 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View