Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

Looking for a manual about NIRS in cognitive science / neuropsychology, suggestions?

Dear community, i'm looking for a manual about NIRS, functioning, methodology and data analysis. in the field of cognitive science / neuropsychology. I would like to know as much as possible...

30 January 2020 8,861 2 View

Could you recommend a book on White Mice brain or on pathology of white mice (Mus musculus) brain?

The book will be used as a reference.

17 March 2018 739 3 View

Does anyone know a replacement for RNA carrier for Qiamp minelute virus spin kit?

I am intended to sequence (via Illumina) viral nucleic acids, including RNA. I was informed that using RNA carrier is not recommended in this case. However, since my samples will have a low...

29 November 2017 8,244 2 View

LPS dosage with LAL QCL-1000 Chromogenic endpoint Lonza, does anyone with experience?

I'll dosage LPS with LAL QCL-1000 Chromogenic endpoint Lonza, and i have many questions about this. Has anyone used this kit yet? Does anyone know if all materials should be depyrogenised? Can the...

03 April 2017 7,719 1 View

Dear colleagues, Can anybody help me identifying these fish vertebrae?

25 May 2016 2,532 1 View

I am starting a research on schools and clubs offering sports. Anyone know any database on the subject?

07 March 2016 9,875 3 View

Where can I find software for data classification using label propagation?

Dear colleagues, I am doing some research in data classification, and I would like to compare to some classification approaches that uses label propagation (see e.g. Zhu, Ghahramani,...

22 October 2014 3,161 3 View

Have you ever had problems with 1.8 K close cycle refrigerator (Joule Thomson valve cold head)?

We are having problems with our JT cryostat. We have cooled down our system three or four times and it easily reached the base temperature (~ 1.8 K). However we cannot cool down the system below...

15 July 2014 8,288 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View