hii all,
i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ?
and how i can ensure that i have a E.coli in log phase?
thank you?
Christopher S Morello
Here's a little more info:
http://jb.asm.org/content/189/23/8746.full
https://www.qiagen.com/us/resources/technologies/plasmid-resource-center/growth%20of%20bacterial%20cultures/
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Gamal Enan
You can get logarithmic growth of E.coli within 12 h by inoculation of actively growing young cells around 1% v/v (3 log ) in Either BHI or LB broths and by incubation at 37 degrees at pH 7.4 and moderate chaking conditions.
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