Log phase of E.coli?

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High Power control for 48V battery pack - High current control?

Hi, I am working on 48V Lithium-Ion battery pack. I am wondering if there is fast MOSFET (or any other technology), to control the current of charging and discharging the battery with high...

20 January 2021 1,717 4 View

GROMACS: Cannot rename checkpoint file; maybe you are out of disk space?

Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...

03 January 2021 3,544 2 View

How can the resistance of paving asphalt be increased to acid rain?

Modification of the rheological properties of asphalt

25 December 2020 7,390 2 View

Can someone please share the matlab code of FBMC/OQAM PAPR reduction with TR and PTS methods?

I am trying to work on reducing the PAPR of the FBMC/OQAM with the TR and PTS methods. for that if someone can share with me one of these matlab codes. my e-mail: [email protected]

19 December 2020 7,764 1 View

Appreciate the quality qualities of cheese ?

How to estimate the acidity of a cheese?

13 December 2020 4,890 4 View

What is the appropriate polymerase to amplify gDNA greater than 10kb?

I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...

03 December 2020 1,939 5 View

How to get the data of the changing the distance between two Pionts with the applied force?

does anybody knows how can i get the change in the distance between the notch nodes (two points showing in the figure ) vs the applied force in every step as a table out put or curve in...

23 November 2020 352 3 View

Why I got low CI in the EBSD?

Hi I got a low confidence index in the EBSED analysis even after days of polishing using 1 to 0.02 um suspension using 1 lb force, the sample was Al/W composite (has intermetsllic interface)...

26 August 2020 3,547 4 View

Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Hi Is it needed to etch a sample in order to see dislocations? SEM or TEM microscope should be used for that?

hi please I have Al/W sample and I am looking for screw dislocations on the intermetallic surface, my question is: Should the sample be etched? and should I use the SEM or TEM microscopy? what...

06 August 2020 9,703 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Christopher S Morello

Here's a little more info:

http://jb.asm.org/content/189/23/8746.full

https://www.qiagen.com/us/resources/technologies/plasmid-resource-center/growth%20of%20bacterial%20cultures/

Gamal Enan

You can get logarithmic growth of E.coli within 12 h by inoculation of actively growing young cells around 1% v/v (3 log ) in Either BHI or LB broths and by incubation at 37 degrees at pH 7.4 and moderate chaking conditions.