Howperixosomes extraction by biochemical technique will be achieve?

Which biochemical technique do you suggest for the separation of Perixosomes?

02 March 2018 9,923 0 View

Which of these two grug suitable for diabete Glyclzide or Pitoz or both together?

People usually take these two drugs simultaneously what is your recommendation?

10 November 2017 6,978 0 View

What is the best method to separate golgi system from the cell ?

In order to separate Golgi apparatus from other cell particles which method should be used?

05 June 2015 7,614 2 View

How iron is taken up my nuclei for synthesis of enzyme containing iron?

I would like to know exact mechanism by which iron is taken up by nuclei/

31 December 2014 4,272 0 View

Is there mitochondria transferrin receptor in the cells?

31 December 2014 5,935 0 View

What is the best treatment for tinnitus?

Tinnitus might be treated by medicine either herbal or chemical.

10 November 2014 559 13 View

Which of these two materials are more toxic: Acrylamide or bisacrylamide? Are they neurotoxic?

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09 October 2014 2,198 4 View

How thought will be made by our mind

where is the source of thought .what is its relation with mind

08 September 2014 9,851 1 View

do you believe in using water purification apparatus at home

water purification system using for home

05 June 2014 1,457 1 View

Is there relationship between Mn toxicity and cathecolamines levels in the brain?

Parkinson like disease should be related to Mn toxicity in the brain.What would be changes in the cathecolaminies levels in the brain?

01 January 1970 4,553 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View