What is the best method to separate golgi system from the cell ?

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Howperixosomes extraction by biochemical technique will be achieve?

Which biochemical technique do you suggest for the separation of Perixosomes?

02 March 2018 10,028 0 View

Liver enzyme assay?

What is the method for tyrosin trasaminase assay?

10 November 2017 4,328 0 View

Which of these two grug suitable for diabete Glyclzide or Pitoz or both together?

People usually take these two drugs simultaneously what is your recommendation?

10 November 2017 7,084 0 View

Is there mitochondria transferrin receptor in the cells?

Is there transferrin receptor on mitochondria membrane.

31 December 2014 6,041 0 View

How iron is taken up my nuclei for synthesis of enzyme containing iron?

I would like to know exact mechanism by which iron is taken up by nuclei/

31 December 2014 4,385 0 View

What is the best treatment for tinnitus?

Tinnitus might be treated by medicine either herbal or chemical.

10 November 2014 667 13 View

Which of these two materials are more toxic: Acrylamide or bisacrylamide? Are they neurotoxic?

09 October 2014 2,333 4 View

How thought will be made by our mind

where is the source of thought .what is its relation with mind

08 September 2014 9,944 1 View

do you believe in using water purification apparatus at home

water purification system using for home

05 June 2014 1,548 1 View

Is there relationship between Mn toxicity and cathecolamines levels in the brain?

Parkinson like disease should be related to Mn toxicity in the brain.What would be changes in the cathecolaminies levels in the brain?

01 January 1970 4,661 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View