Hi i have some question in regards to this kit. My samples contains a blend of micro-algae and bacteria. I would like to to use this kit to quantify the dead/ live cells in my mixed liquor. However based on some papers of the absorbance wavelength of micro-algae is similar to that of the 'dead' cells of the kit. (reference to the paper below)
1) In this case is there any interference of using this kit, if my mixed liquor contains high amount of micro-algae?
2) Is it possible to isolate purely the bacteria i my mixed liquor without algae and vice versa?
3) Any advice on how to differentiate between bacteria (live and dead) vs algea vs fungi?
There is no way to discriminate the two populations according to the red fluorescence signals. Chl autofluorescence and PI emission spectra overlap. However, there should be consistent morphological differences between micro-algae (generally bright large cells) and dead bacteria (generally smaller than microalgae), as they appear at the epifluorescence microscopy. If using flow cytometry, side and forward scatter signals still offer some opportunities for correct discrimination.
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