Hi all, is it possible to perform a Live /Dead assay on fixed tissue using Pi and FDA or any other dye?
Thank you in advance
Thanks Christina, how thin do you think it can be my sample? would you suggest to use Calcein AM instead of FDA?
I was trying to create a FEM model of an LVI and CAI experiment. For the CAI simulation, the load displacement curve I obtained is way too stiff compared to the experimental result. The maximum...
06 July 2023 1,742 6 View
I'm currently working on samples of Formica rufa group that where caught on the Italian Alps probably around ten/fifteen years ago. Some of the test tubes show ants preserving a normal...
02 March 2023 7,942 3 View
Any tips would be highly appreciated. The diffractometer is a Bruker D8 Advance. My first thought is to mount a UBC collimator and set the sample height with a UMC sample stage (XYZ...
08 January 2023 3,951 5 View
Is there a tool to determine if a dialysis patient needs ambulance transport? Is there a tool that allows me to understand the degree of autonomy of the dialysis patient? The goal is to evaluate...
27 May 2022 9,191 7 View
I want to know if my supposition is possible and achievable, or if it is impossible. I was considering that colors are just reflection of the light toward the eye's retina. It is the case because...
16 April 2022 1,856 6 View
I'm currently working on ball-on-disk tribological test using different oils. Is there a direct relation between the coefficient of friction and the lubrication regime of the coupled surfaces? Is...
27 March 2022 4,783 5 View
Our university project group is studying the possibility to apply a plan for the (local) restoration of ice based habitats of polar bears (Ursus maritimus). Has the idea already been developed in...
29 November 2021 6,029 2 View
Does anyone know if there is a dedicated plugin for QGIS for browsing STAC catalogue datasets? Seems that once there was one, but now is not anymore...
22 November 2021 7,573 3 View
Hi, is there anybody who can share a good protocol for cell membrane (transmembrane) protein extraction from zebrafish brain tissue to be assayed by Western.? I tried one with a soft lysis...
08 June 2021 8,823 3 View
Hi everyone, I know the presence of a tail spine isn't a common feature for Mobulidae, but i cannot find any image / description regarding the argument, both for living or extinct specie. Any help?
28 May 2021 6,990 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View