To encapsulate dye in liposomes, dissolve lipids and the dye in a solvent, create a lipid film, hydrate with buffer, and sonicate or extrude to form vesicles, then remove the unencapsulated dye. To generate liposome potential, incorporate ions in the internal phase, measure the transmembrane potential, and expose it to non-ionizing radiation while monitoring changes in dye release or fluorescence.

Encapsulation of Dye in Liposomes

Encapsulating a dye in liposomes involves preparing the liposomes and loading the dye into the aqueous core or lipid bilayer, depending on the dye's hydrophilicity or hydrophobicity.

Here's a detailed procedure with examples of ingredients that can be used:

Materials

Procedure

Creating Potential in Liposomes

To create potential across liposome membranes, a transmembrane ion gradient is established, which mimics cellular membrane potentials.

Procedure

Assessing the Effect of Non-Ionizing Radiation