Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

Are you a virtual team member/leader who would like to participate in a Delphi study on International Online Collaboration Competencies (IOCC)?

You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...

11 November 2020 9,715 1 View

Bonferroni or Tukey: Which post-hoc test is adequate for my data?

Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...

21 October 2020 2,182 5 View

Does anyone know a good paper about deep watershed application for XCT images of rock?

I was able to find some applications of deep watershed for image instance segmentation, but nothing for rocks. Could you please recommend any study?

07 September 2020 5,731 8 View

What are reaction conditions for aromatic sulfonation of a dibenzyl ether derivative?

I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...

04 August 2020 4,446 7 View

Can you recommend any publications or authors on ethical and eco-friendly marketplaces?

Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon

27 July 2020 7,302 3 View

What are your nanoindentation tips cleaning tricks?

Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...

08 July 2020 5,926 5 View

How good is the quality of spectra taken by portable NMR spectrometer?

At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...

22 June 2020 8,144 6 View

Commercial materials/surfaces for contact measurement?

Does anyone know of, or use, commercially produced surfaces for contact measurements (control material)? As an example, is there a producer of PTFE discs with highly controlled surface roughness...

09 April 2020 8,806 5 View

What are the pros and cons of running virtual conferences?

What are the pros and cons of running virtual conferences? I ask this question because the current challenging situation with the COVID-19 pandemic has, understandably, meant the cancellation or...

27 March 2020 8,346 17 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View