Hello Every one, I am wondering whether southern blot can determine the mutated genes while they will be used as probes. Will it show result if the DNA fragment used as Probe has mismatches at intervals. Secondly, If the probe is 1KB long, but 700 bp is same, but 300 bp are not same on either of the sides. Will southern blot give the result or not? If yes, what is the possible solution to get a accurate band. Thank you. 

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