Muhammad Safiullah Virk

4 Questions 6 Answers 0 Followers

Questions related from Muhammad Safiullah Virk

Limitations of Southern Blot?

Hello Every one, I am wondering whether southern blot can determine the mutated genes while they will be used as probes. Will it show result if the DNA fragment used as Probe has mismatches at...

04 May 2017 3,331 2 View

How can mutations be avoided during PCR?

Every time we run the PCR, Especially for protein expression etc, we fine mutations in out fragment which dont let our protein to be expressed. What are the ways, conditions, chemicals to avoid...

22 December 2015 8,243 6 View

What is fate of introns during splicing?

The main question i want to know is, is every intron have its own machinary yo be removed or machinary for intron control is some where upstream of the gene? Secondly, bioinformatic analyses for...

22 December 2015 1,338 1 View

What is the fate of suicide plasmid in fungi gene knockout or gene silencing? Does it keep growing with generations or is it removed?

Hello Everyone, I need to know about the difference of fate of suicide plasmid in fungi and in bacteria.. As I know, in bacteria, suicide plasmid deletes a gene and is removed in next generations,...

09 December 2015 7,728 3 View