Lettuce seed strelization?

More Melisa Türe's questions See All

How can I get RAW264.7 cells to differentiate into osteoclasts?

I can't get RAW264.7 cells to differentiate into osteoclasts. I've tested different densities: 5000 cells/cm2 , 10,000 cells/cm² and 20,000 cells/cm² in 35mm polystyrene. I use DMEM medium, 10%...

12 June 2024 4,604 1 View

Is anyone experiencing the same problem with RAW264.7 cells?

Hello, I have been cultivating RAW264.7 cells from ATCC recently. I culture them in DMEM medium + 10% FBS at a density of 10,000 cells/cm² in a T75 flask with a volume of 10mL. For cell...

27 February 2024 4,487 2 View

What types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans?

I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...

21 February 2024 7,127 1 View

Merhaba değerli hocalarım ve arkadaşlarım. Tezimde çalışmam gereken Mycobacterium suşlarımda eksiklerimi tamamlama konusunda yardımcı oluyor musunuz?

Merhaba değerli hocalarım ben Melisa, Mersin Üniversitesi Moleküler Mikrobiyoloji bölümü yüksek lisans yapmaktadır. Tezimde Mycobacterium türlerini çalışıyor. Bütçenin kısıtlı olmasından dolayı...

20 August 2023 2,188 0 View

Merhaba değerli hocalarım ve arkadaşlarım. Tezimde çalışmam gereken Mycobacterium suşlarımda eksiklerimi tamamlamam konusunda yardımcı olur musunuz?

Merhaba değerli hocalarım ben Melisa, Mersin Üniversitesi Moleküler Mikrobiyoloji bölümünde yüksek lisans yapıyorum. Tezimde Mycobacterium türlerini çalışıyorum. Bütçenin kısıtlı olmasından dolayı...

31 May 2023 9,288 0 View

Is there a way to remove Alizarin Red staining off sample?

Hello, I have an issue with my Alizarin Red staining. It gives random unspecific positivity stains throughout the tissue (Embedded in paraffin, dewaxed in Xylene). My question is, how do I remove...

11 December 2022 9,941 2 View

Did my L929 fibroblast cells transform into IPCS ?

This is the first time I studied induced pluripotent stem cell. I used a non-viral vector conjugated with a plasmid (encoding :Oct4, Sox2, Klf4 v-Myc and GFP) to transfect L929 Fibroblast cell...

08 January 2018 240 7 View

Hi! I'm having trouble with the isolation of intracellular parasites, i need a technique for degrading host cell without damaging guest cell

Hi there ! I'm looking for some protocol with which I can use the whole cell culture (blood) and break down the cell membrane of the host cells to release the parasites, but I need the guest cells...

24 June 2017 7,773 1 View

Has anyone done Immunofluorescence of PFA 4% fixed spleen sections?

I am using the following protocol: 1) Fix the spleens with PFA 4% overnight 2) Put them into a saccharose solution of 15% over night at 4ºC, or until they sink. 3) Put them into a saccharose...

11 May 2017 2,906 5 View

How to stabilized biofilm in RNAlater (Qiagen)?

Hi, I am trying to extract RNA from Pseudomonas aeruginosa biofilm adhered to falcon tube. Some protocols that said the biofilm should be washed with PBS and then resuspended in RNAlater. I have...

04 October 2016 9,834 3 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,300 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,897 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,667 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,143 2 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

Mansoure Hatamian

it depends on the concentration

more concentration, then use less time

Paul Reed Hepperly

10% commercial Clorox bleach sodium can remove the external contaminants with a 4 minute treatment. The chemical residue can be removed by riinse in sterile distilled water for one minute. The drying and ventilation provide a surface disnfected seed. This will not control internally seed borne fungi and bacteria.

Melisa Türe

Mansoure Hatamian Thank you.

Paul Reed Hepperly Thanks a lot Sir

Girma Hailemichael Gebre

The following links can help you read and understand about Lettuce seed: https://harvesttotable.com/lettuce-seed-starting-tips/https://academic.oup.com/jxb/article-abstract/34/5/620/453326?redirectedFrom=PDF

Thank you!