Hi! I'm having trouble with the isolation of intracellular parasites, i need a technique for degrading host cell without damaging guest cell

Hi there ! I'm looking for some protocol with which I can use the whole cell culture (blood) and break down the cell membrane of the host cells to release the parasites, but I need the guest cells...

24 June 2017 7,615 1 View

How to stabilized biofilm in RNAlater (Qiagen)?

Hi, I am trying to extract RNA from Pseudomonas aeruginosa biofilm adhered to falcon tube. Some protocols that said the biofilm should be washed with PBS and then resuspended in RNAlater. I have...

04 October 2016 9,678 3 View

How to find average particle size using Langevin fit in M-H curve?

08 May 2016 8,564 25 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How to extract and purify disaccharide peptides and lactoyl peptides from Mycobacteria cell wall?

Recently, I try to obtain disaccharide peptides and lactoyl peptides from Mycobacterium Smegmatis, but I cannot succeed. My method is: Disaccharide peptides: The mycobacterial strains were...

12 January 2021 8,352 3 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

Does anyone know one antibody that works against Mycobacterium spp?

Anti-Mycobacterium antibody: does anyone know one antibody that works against Mycobacterium spp. (mainly for immunofluorescence)? Many thanks in advance! Cristiane

04 January 2021 9,724 3 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View

Are there new ways or new techniques in culturing or isolating Mycobacterium leprae?

I have read from books that Mycobacterium leprae is only grown in foot pads of mice and armadillo, so I have been wondering if there are newly developed techniques in isolating and/or culturing...

03 October 2020 6,065 2 View

Hi all, I need to transform a reporter plasmid into a mutant of Mycobacterium avium hominissuis which has the same antibiotic marker as the plasmid?

I tried to electroporate and select the colonies by plating onto a higher concentration of antibiotic. But it did not work!

20 September 2020 6,458 3 View

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View