Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

Statistics: Controlling for pubertal status and gender?

Hi, I am not sure whats the best way to "control" for pubertal stage and gender in my analysis. Actually, puberty is classified in 5 stages (1=prepubertal to 5=fully grown). To make it easier and...

06 May 2020 9,363 3 View

How to make a 1500 watt high voltage(5-20KV) power supply?

I need to design and make a high voltage power supply to use in a lab made electrostatic precipitation with this specs: Input voltage: AC: 220 volt Output voltage: DC: 5-20 KV (Continuous...

09 April 2020 5,172 21 View

Experience with immunostaining with anti-Hepatocyte Specific Antigen (Hep Par-1) antibodies in frozen tissue?

Dear scientists, I would like to stain the formaldehyde-fixed, OCT-embedded human liver tissues with anti-Hep Par-1 antibodies (DAKO; clone OCH1E5) for IHC staining. Do you by any chance having...

20 February 2020 3,017 3 View

What causes wrinkles in thin films to disappear after heat treatment?

When I anneal the free standing thin film (>300 degree Celsius), wrinkles of the thin film disappear. What is the principle of this phenomenon? (This thin film is can be a single layer or a...

28 December 2019 8,410 4 View

What happens to the mechanical properties when annealing a metal thin film?

If the metal thin film is annealed, How do the mechanical properties such as tensile strength, hardness, Young's modulus change? The metal thin film is not alloy and thickness is less than 100...

21 August 2019 8,069 3 View

How do you calculate IC 50 for inhibition of tyrosinase activity?

How do you calculate IC 50 of different plant extracts in tyrosinase inhibition essay?

28 April 2019 8,241 2 View

Induce Point Mutation in Microbes (RpoB gene for Rifampicin resistance)?

Hi, I try to make Rifampicin resistance microbes (e.g., Corynebacterium) using point mutation in RpoB gene. The doubling time of Corynebacterium is ~3.5hrs, and spontanoeus mutation rate for...

01 April 2018 6,253 2 View

Possible reasons causing ribosomal RNA size shift?

Hi all, I ran my RNA gel (formaldehyde gel containing MOPS buffer) to check the integrity of ribosomal RNA isolated from primary mouse hepatocytes. The 18s and 28s bands are good, which may...

26 November 2017 5,882 1 View

CDNA synthesis of norovirus

04 August 2017 2,580 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Business strategy research thesis topics for IIoT Platforms?

Hello, I am a Master in Management student currently interning at a leading Industrial Automation company. I need to do a business strategy thesis which brings together the fields of IIoT...

04 February 2021 7,315 3 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Is it possible to economically reuse the heat content of water vapour that is generated during quenching of continuous casting of steel?

A huge amount of water is evaporated to cool down and solidify the liquid metal strands coming out of bottom of CCM mould. The steam contains significant latent and sensible heat energy, besides...

26 January 2021 3,446 4 View